非常有启发意义的审稿人意见：编辑说明

e and temperature of incubation steps; the cell densities used for cell culture experiments; and any other necessary details that are required for replication. The current methods section is insufficient for this purpose.

译本：①所述了三维建立年前动物的绝育和脑参杂分析方法。②染色解决方案理应所述腹腔的药剂、美味许多组织的运应用于和石蜡包埋许多组织的运应用于，但并未具体这些是否都是有所不同的抽样，或者是否为每种处理方式保留了一些抽样，或者脑部是否被分成各不有所不同部份已充分利用所有这些生产力。目年前尚不相符腹腔在切除后如何处理方式以发挥作用此处所述的所有分析方法。③特地指明运应用于的所有试剂盒，包含制造商的资讯、时间、湿度和离心速度、孵育时间和湿度；应用于细胞内培养试验中会的细胞内体积；以及拷贝所需的任何其他必要或许。④当年前的分析方法部份不足以发挥作用此目标。

修改发表意见：材料和分析方法部份理应包含充分的或许，以供到时的分析人员在需要时拷贝试验中会。

#5

研究专家发表意见：Treatment with benzalkonium chloride may not be the best method for removing enteric ganglia, as it is likely very non-selective and may damage numerous enteric components.A more appropriate alternative may have been a transgenic or knockout mouse model of Hirschsprung’s disease, such as the Ret gene knockout, which appears to be the model that best mimics the disease in humans.

译本：用苯扎氯铵放射治疗意味著会伤害多种肾脏化学物质，不是去除肾脏脊髓节建立先天性象腹腔性疾病三维的最佳分析方法。运应用于先天性象腹腔性疾病遗传工程或基因敲除小鼠三维（如敲除Ret基因）格外适合仿真人类哮喘。

修改发表意见：①通过遗传工程分析方法可建立尖锐动物品系以及人类有所不同哮喘的抗病毒在流行病学年前分析中会运应用于格外有劣势。②遗传工程抗病毒不致于放射治疗/药物干预三维与人类某种性疾病状相似的哮喘在致病因素、必要等总体不尽有所不同的问题。因此，运应用于遗传工程或基因敲除小鼠三维可格外好仿真先天性象腹腔性疾病。

#6

研究专家发表意见：This is a preliminary study investigating the use of NEUROD1 overexpression to induce the reprogramming of rat spinal cord reactive astrocytes to neurons, and exploring some possible mechanisms of this reprogramming. One main limitation of the study is that, although 11 proteins were identified as candidates in the bioinformatic analysis, only two were investigated further. In the future, it would be interesting to see western blot results from the other nine candidate proteins, as well as results from functional studies.

译本：这是一项先期分析，分析运应用于NEUROD1过解读诱导大鼠脑部反理应性心形质细胞内重为演算为脊髓元，并聚焦这种重为演算的一些意味著必要。该分析的主要相比较在于，尽管在生物的资讯学分析分析方法中会鉴定出11种细胞内内为候选细胞内内，但仅对其中会2种候选细胞内内顺利进行了进一步分析分析方法。不曾来可就另9种的结果及功能顺利进行聚焦。

修改发表意见：在生物的资讯学分析分析方法中会意味著想得到许多相异解读的细胞内内，理应清晰陈述同样其中会一种或几只相异解读细胞内内的因素，对不曾同样的细胞内内也理应在辩论中会顺利进行陈述。

#7

研究专家发表意见：Given that the authors were investigating proteomic changes associated with the reprogramming of astrocytes to neurons, did they consider looking at the proteomic differences between the GFP group vs. only the DCX-positive cells in the NEUROD1 group (rather than all cells – because the majority of cells in this group did not seem to be reprogrammed)? It seems that this approach would provide more specific findings, and should perhaps be considered for future studies

译本：重新考虑到分析与心形质细胞内重为演算为脊髓元相关的细胞内内质组学变化，是否理应所述GFP组与NEUROD1组仅DCX阳性细胞内的细胞内内质组学相异（而不是所有细胞内，因为大多数细胞内似乎并未被重为演算）？这意味著会提供格外恰当的发掘出，毫无疑问可重新考虑运应用于于不曾来的分析中会运应用于。

修改发表意见：DCX阳性细胞内在评论中会是指真正NEUROD1 转导的心形质细胞内，重新考虑到了并未重为演算的细胞内，证明了的结果将格外能充分体现与心形质细胞内重为演算为脊髓元相关的细胞内内质组学变化。

#8

研究专家发表意见：The original paper was correctly structured but out of balance. The Introduction was too long and the discussion rather short. Please cut down the Introduction as much as possible without altering the content. Discussion could be fuller, perhaps combining the authors’ earlier work with the present results to discuss how the research should develop.

译本：原始研究成果形态错误但不抵消。导言太长了，而辩论则很短。特地在不改变内容的前提尽意味著地缩减导言。辩论可以格外充分，毫无疑问可以将作者既往的分析与目年前的结果结合一起以辩论此分析不曾来的方向。

修改发表意见：理应抵消导言和辩论的内容。导言理应向读者简介分析的氛围的资讯，以及顺利进行试验中会的因素。在导言之后理应相符而错误地陈述此次分析的目标。辩论部份则主要是对试验中会结果顺利进行解释，并简要所述分析的相比较，向和读者简介已经重新考虑到的不足之处，同时在辩论之后理应再次陈述试验中会的主要推论。

#9

研究专家发表意见：Rutin is initially tested from 0–100 µg/mL. The reasons for focusing on 25 and 50 µg/mL treatments are not clearly explained. Please clarify why these specific concentrations were chosen for later experiments.

译本：芦丁原本的测试之内是0–100 µg/mL，有恰当解释为何早先试验中会同样了25和50 µg/mL。特地陈述同样特定pH的因素？

修改发表意见：可在结果中会BV-2细胞内存活检测部份比较各不有所不同pH芦丁的效果后，证明了试验中会运应用于的特定pH为25和50 µg/mL。

#10

研究专家发表意见：The anatomical investigations were quite intricate so it was unsurprising that they could not deliver a large statistical validation. The authors might propose using other techniques (such as histological tracing) that might deliver greater n.

译本：生理学分析是非常复杂的，因此意味著未提供大抽样的统计学验证，但可在许多组织学示踪等其他技术运应用于格外大的抽样量。

修改发表意见：虽然不会顺利进行大抽样的生理学分析，但是理应详细所述此处分析中会给予的结果，并与既往分析顺利进行对比。

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